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Earlier studies indicated that the D- threo-enantiomers of PDMP and structurally related compounds represent the active forms of these GSL synthesis inhibitors [ 18– 23].
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This results in structures that can possibly represent the active form of the receptor.
This orientation might not necessarily represent the active form since no data are available for the reduced protein.
Each node is taken to represent the active form of the protein it is named for.
Alternatively, although much less likely, the lower molecular weight gelatinolytic band could represent the active form of MMP-1 (45 kDa) or MMP-13 (41 kDa), which are capable of cleaving gelatin, albeit with the very low activity (Snoek-van Beurden and Von den Hoff 2005).
Another 35 molecules representing the active forms of CLOUD prodrugs ensure the usability of the library for both biochemical and cell-based assays.
The activity measured in the absence of Glc-6-P represents the active form of the enzyme (I or a form), whereas that measured in the presence of 6.6 mM Glc-6-P represents total GS activity.
The authors suggest that this represents the active form of RBX1 and that other RING E3s may work in a similar manner.
The latter enantiomer probably represents the active form of the compound.
The P subscript represents the phosphorylated form and an A as superscript represents the active form.
The reduced cysteine represents the active form, as opposed to the inactive oxidized cystine dimer.
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