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Values represent stimulation index mean ± SEM.
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Open bars represent stimulation in the absence of IFNγ and solid bars stimulation in the presence of IFNγ.
The proliferation of splenocytes was represented by the stimulation index (SI): SI = mean absorbance value at A450 of stimulated cells divided by the mean absorbance value at A450 of the negative control.
The stimulation index represents the ratio of mean counts obtained in the presence of antigen to mean counts obtained without antigen.
Stimulation index.
The stimulation index (SI) was calculated using the following formula: Stimulation index (SI) = OD of stimulated cultures OD of unstimulated cultures.
In both groups, the stimulation index of SAPNF-treated groups were about two times the controls.
The stimulation index was calculated as described [22].
The stimulation index correlated with cell death only with CD3/28 stimulation (Figure 6D, r = −0.3371, p = 0.0334).
A stimulation Index greater than 1.25 indicates that there was a significant response to Ag stimulation [10].
Stimulation index was calculated by dividing the MOG35 55-specific MOG35 55-specificproliferation in cultures with medium only.
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