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We typically represent fluorescence lifetime data in fluorescence lifetime "images" for which a false colour scale encodes the lifetime parameter and the brightness encodes the total fluorescence intensity.
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The panels represent fluorescence by the chemical elements calcium, iron, mercury and lead (from left to right).
As the FPD only represents the average fluorescence lifetime, it is limited to the approximation of single-exponential fluorescence decay theory.
We show that the FPD, which represents the average fluorescence lifetime of fluorophores at any given detection bandwidth, is inherent to all cytometry systems and proved this by simulation and evaluation with different algorithms.
Nonetheless, they can be differentiated with fluorescence lifetime analysis [ 16].
The fluorescence lifetime measurements were represented in two different ways.
However, when co-transfected with mOrange-CDC25B-WT or mOrange-CDC25B C487S mutant, this value was reduced to 2.101 and 2.086 ns respectively (representing 14.7 and 15.3% variations in fluorescence lifetime).
The chlorophyll fluorescence lifetime can be estimated from deconvolution of the fluorescence and background signals.
Fluorescence lifetime imaging could be performed during 3D scanning.
The AUC values represent the probability that a randomly chosen pixel in a cancerous region has a greater fluorescence lifetime or fluorescence intensity than a randomly chosen pixel from a non-cancerous region.
The longer fluorescence lifetime measured at 610 nm in cells treated with the cholesterol/cyclodextrin mixture represented the state of the dye in the membrane lipid disordered phase.
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