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Reposition time!
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Since the position control for repositioning the gimbal axis takes time, the axial drift compensator is designed for the nonlinear drift model in the inverse dynamic control framework to shorten the repositioning time.
The cynic will say that Powell has discovered that it's repositioning time in America.
A larger AOD can be used to get more resolvable points, and therefore a larger diffraction-limited field of view, minding the increased repositioning time.
Nucleosome repositioning time courses were simulated using a Monte Carlo program written in C++.
In the first set of simulations, repositioning time courses at a single fraction of bound ISWI were generated and analyzed.
All repositioning time courses were globally analyzed using eqs 1 and 2 as described in Experimental Procedures.
Analysis of repositioning time courses using eqs 1 and 2 and ATPase time courses using eq 3 was performed using Conlin.
We next performed global analysis of the repositioning time courses, which includes both nucleosome substrates, F18N18F and F24N24F, together at each ISWI concentration.
We then performed additional global analysis, including all of our nucleosome repositioning time courses together with these species fractions as additional constraints, to determine the repositioning activity of each species.
We also globally analyzed the repositioning time courses for F18N18F and 24N24F together at several ISWI concentrations and found that the rate of repositioning increased with an increase in ISWI concentration as expected.
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