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What is the reporting sequence?
Our findings strongly suggest that, despite many recent papers reporting sequence convergence in particular candidate genes, the importance of this mode of molecular evolutionary change is relatively underappreciated, and is under-exploited in seeking to understand the genetic basis of complex traits such as echolocation.
The drug packets were administered to patients serially according to the patient's reporting sequence.
The IARC Working Group suggests a five category system for reporting sequence variations: pathogenic, likely pathogenic, uncertain, likely neutral, and neutral [ 18].
Similarly, the Dutch and British societies for clinical molecular genetics suggest reporting sequence variants in five classes: (1) clearly not pathogenic; (2) unlikely to be pathogenic; (3) unknown significance (VUS); (4) likely to be pathogenic; and (5) clearly pathogenic [ 25].
RUNES categorized each variant according to ACMG recommendations for reporting sequence variation [ 18– 21] and with an allele frequency (MAF) derived from CPGM's Variant Warehouse database of approximately 90 million variants and 3,900 individuals [ 6, 11, 12].
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Also, articles reporting sequencing results, such as publications from The Cancer Genome Atlas project, were excluded.
We added a note on this: "Also, articles reporting sequencing results, such as publications from The Cancer Genome Atlas project, were excluded.
Subsequent analysis based on whole-genome sequencing discovered two changes to previously reported sequence types and the presence of plasmid replicons in three strains (Supplementary Table 4).
We sequenced the promoter region of the RHCE gene, and compared our results with the reported sequence.
The MLST of each wild-type strain was confirmed compared to the original reported sequence type32 for all but two strains, K56-41 and K56-70.
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