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Moreover, relaxation dispersion data from residues reporting on processes III and IV are all well fit to a two-state model.
There was also insufficient reporting on processes for dealing with missing data in many studies and this further restricted the studies' generalizability.
To further confirm that CEST profiles observed for residues reporting on processes I and II are not the result of direct Zn binding we added ∼140 μM Zn to a Zn and Cu-free apoSOD12SH sample in a Suprasil tube.
(D ) This is illustrated further by comparing calculated Rex values for residues selected as reporting on processes III (green) and I (red) using Equation 1 of 'Materials and methods' and the best fit exchange values and chemical shift differences.
CPMG and CEST data sets recorded on pWT apoSOD12SH and reporting on processes I and II could not be fit simultaneously to a two-state model of chemical exchange.
(E ) Comparison of pE (fractional population of the excited state) and kex (sum of forward and reverse exchange rates) values obtained from separate two-state fits of dispersion profiles reporting on processes I (red) or III (green) (1.5 mM G85R apoSOD12SH, 600 + 800 MHz, 25°C) showing that each process can be well fit to distinct exchange parameters.
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Reporting on specimens processed from the district and hospital laboratories is integrated and submitted along with the weekly disease reports for selected priority diseases.
Introducing automated workflows to eliminate repetitive, manually intensive processes will simplify data gathering and analysis, improve communications and productivity levels and facilitate accurate tracking and reporting on process steps and completion dates.
Amino acids localized to the electrostatic loop (residues 130 140) were thus selected as reporting on process II (Table 3).
This value is clearly much smaller than those calculated and measured for residues reporting on process III.
(B ) Residues identified as reporting on process III via CPMG experiments (see 'Materials and methods') are highlighted on the structure of a monomer of Cu2Zn2SOD1S S.
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