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At present, all studies reporting copy number variants (CNVs) in patients with POI by array-CGH have used lower resolution platforms (Aboura et al., 2009; Ledig et al., 2010; Liao et al., 2011).
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It then reports copy numbers for each segment, and flags segments that exceed the thresholds for gain and loss calculated by the model.
In B. subtilis, pBAV1K-T5-luxABCDE replicates to 10 times the reported copy number of pGK12 in the same species [5].
The multiplex assays were repeated, without variation, up to four times for each sample, and real-time quantitative PCR reported copy number consistent with the ability to repeat the multiplex results.
Zhang et al reported copy number losses of mir-218-1 mir-218-1 mir-218-1n (16%), breand (36%) and melanoma (33%), however, the alternative precurSLIT2nd host gene were not mentinned [48].
We report copy number gains that were detected in chromosomes 4, 7, 11, 12, 14q, and 18q.
This suggests that the 65000 to 38000 range in previously reported copy numbers [ 31– 33] reflects differences in antibodies and methodology.
Three studies have reported copy number changes in EOC [ 16, 17, 24] (Additional file 4: Table S2).
Furthermore, TOP2A gene locus at 17q21-22 has reported copy gains and overexpression, and regulated chemo-resistance in HCC [ 47].
As a final output, it does not report copy neutral LOH which is important in studies of cancer.
We report copy number variation among the most recently duplicated Abp genes, which suggests substantial volatility in this gene region.
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