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The rME reporter strongly activated in 100 nM T3 in the retina with the exception of a notable AP-negative patch in the central-nasal part of the retina, which was observed in all T3 conditions (Fig. 6A-D).

However, observing a variety of distributions for different insertion sites of the reporter strongly suggests that the major source of noise is intrinsic.

Importantly, the NELF-dependent expression of these reporters strongly suggests that the generation of a productive Pol II elongation complex is the key step in the transcription cycle that is targeted for integrating the regulatory cues that drive the patterned expression of these genes in the early embryo.

We examined other 3′-UTR reporters strongly repressed by OMA-1/ 2 to see if they are also repressed by LIN-41 in oocytes.

Taken together, the activity of Atf7ip depletion against three Xi reporters strongly supports a role for Atf7ip in the maintenance of the Xi.

In parallel, the level of the reporter protein strongly increased between these times.

As expected, T3 activates the reporter more strongly than T4.

Evidence for this comes from data showing that a NF-κB reporter is strongly activated in muscle fibers at 3, 7, and 10 days of disuse by hind limb unloading [5], [7], [8].

In the presence of FAK, Dvl2 and wnt3a the reporter was strongly induced (Fig. 6B), and this signal was more than doubled in the presence of the β1 integrin.