Sentence examples for reporter sequences from inspiring English sources
See Supplementary Tables 1 and 2 for primer and C9RAN NLuc reporter sequences.
A carboxy-terminal 3xFLAG-tag was included for western blot detection and a precision protease (PSP) cleavage site was introduced between the repeat and reporter sequences to allow for efficient release of NLuc from the DPR.
The single-stranded oligonucleotide probes were hybridized to dye-labeled (Alexa Fluor 647) reporter sequences, which were provided for a FRET-sensitized emission signal due to proximity of the QD and dye.
We cloned dfoxO 5'UTR A, B and C between protein-encoding reporter sequences of a di-cistronic construct [28] (Fig. 2D I).
Whole genome expression data were generated for all F2 progeny for four different tissues (gonadal adipose, liver, kidney cortex, and kidney medulla) using a custom Agilent mouse array containing ∼40,000 unique reporter sequences.
Plant selection, expression, transformation and growth The reporter sequences were shuttled into the plant expression vector pPTbar, a derivative of the pPZP212 vector [34] containing the BASTA resistance, a 35S-promoter and a rbcs-terminator.
To analyse the regulatory mechanisms controlling hBUB3 gene expression, we characterized the 5′-flanking region from nucleotide −1.3 to +0.58 kb by cloning various potions of this region in front of a luciferase reporter sequence.
In this study, the ability of various cell types to activate P4, independent of the other virus host interactions, was examined in transgenic mouse lines bearing a β-galactosidase reporter sequence driven by the P4 promoter.
Therefore, the potential for adgf-a gene UTR-mediated post-transcriptional regulatiof of the GFP reporter sequence exists.
Each reporter sequence was individually considered as a trait for each tissue and used to map expression QTL (eQTL).
For exon -2a the following primer sequences were used; forward primer 5'-TCGCGCCTAGCCTTGG-3', reverse primer 5'-GGCATCAGCGGGCTTCA-3' and FAM reporter sequence 5'CTCAGCTCCTTAGATGTCGGTC-3'.
