Co-transfection of ERG and Id2 reporter resulted in an increase in luciferase activity.
Upon infection, Tat production from the integrated provirus leads to activation of the lacZ reporter, resulting in synthesis of beta-galactosidase in these cells.
As evident in Fig 5B, co-transfection of Myocardin with the "m-m-wt-luc" reporter resulted in dramatic promoter activity increase.
Strong induction of luciferase was observed when the Gli-BS reporter was co-transfected with a Gli1 expression plasmid, whereas the mGli-BS reporter resulted in background levels of induction (Figure 6A).
Transient exposure of human sarcoma cell line, containing Cre-dependent lacZ reporter, resulted in efficient recombination in the host chromosome, indicating that the bacterially delivered protein was not only efficiently localized to the nucleus but also retained its biological function.
The inverse reporter resulted in a slight decrease in miR-132 levels that did not reach statistical significance (0.77±0.22 fold; p>0.9 ANOVA with Tukey post-hoc; n = 2 cultures per condition).
This is particularly evident from the failure of heat shock induced HLH-1 to activate any of the enhancer reporter genes; this enhancer reporter resulted in strong, widespread, ectopic activity in embryos when used with a known HLH-1-responive enhancer in a previous study [7].
Data were combined such that endorsement by either reporter resulted in a positive diagnosis.
To monitor Azot expression, we designed a translational reporter resulting in the expression of Azot::dsRed under the control of the endogenous azot promoter in transgenic flies.
The co-transfection of miR-194 with a WT reporter resulted in a highly significant decrease in luciferase activity compared with the control-transfected group.
