Fusion reporter methods are important tools for biology and biotechnology.
A variety of assays can be envisioned that monitor either the biochemical activity of apoptosis proteins, such as caspases, through various reporter methods or where ectopic expression of mammalian apoptosis proteins in yeast results in an easily scorable phenotype, such as cell death.
We used promoter J23101 as a reference standard for all relative promoter measurements by dividing each measured activity (Figure 3) by the activity of promoter J23101 for the same reporter (Methods).
Objectives: We used cell-based reporter methods and informatics tools to efficiently screen a large collection of environmental chemicals and identify compounds that induce oxidative stress.
Although both standard and reporter methods have been useful in evaluating RNAi efficacy, a significant limitation is that they are generally applied to populations of cells.
To reduce the high false positive rate of single-reporter method, a double-reporter RGMS vector was configured, in which an xylE-neo double-reporter cassette was used to monitor ccaR expression; 90% of mutants selected by the modified method showed improvement in CA titer.
Subsequently, a double-reporter circuit was configured, in which a xylE-neo double-reporter cassette was used to monitor ccaR expression in order to reduce the high false positive rate of the single-reporter method.
