Sentence examples for reporter marker from inspiring English sources

In contrast, our initial design of the reporter marker consisted of a promoter and promoter-less gene.

Plasmid pCORE with KlURA3-kanMX4 counter-selectable reporter marker cassette (CORE) and plasmid pSH91 were provided by Francesca Storici [ 18] and Thomas D. Petes [ 19], respectively.

One important feature in this system is the reconstitution of the reporter marker composed of two truncated 5′ and 3′ yeast URA3 genes spilt by a modified yeast actin intron.

Co-cultivation period, type of explants, a gfp gene as reporter marker to allow early stage selection of transformants and selection regime were critical factors for successful transformation of Impatiens.

A more recent study showed overlapping expression of nanog with GATA-6 and a Pdgfra reporter, markers of the primitive endoderm lineage, from the morula to the 64 cell stages [8], suggesting a gradual transition from a pluripotency program to a committed state.

For S96, a CORE cassette (Storici et al. 2001) (kindly provided by Michael Knop) with KlURA3 (counterselectable) and kanMX4 (reporter) markers were inserted by standard DNA-targeting procedures (Gietz and Schiestl 2007) at the respective ORF locus.

Before screening the Hela cDNA library, the inability of CD40 to activate the transcription of the reporter markers was confirmed as EGY48 yeast cells harbouring the pEG202/ LexA-CD40 bait construct were unable to grow on Glu/CM-H,L plate.

To accompany the docking lines, we describe a kit of insect transgenesis plasmids allowing efficient multiple-insert cloning and harboring either of seven possible transgenesis reporter markers, including a novel puromycin resistance cassette for transgenic larva selection in antibiotic-containing water.

Another approach would be to generate cell lines specifically expressing only one of the SA species and a reporter gene marker (e.g. GFP, mCherry), co-culturing them and using FACS to assess the capacity of various strains to infect both types in single and multi-cycle experiments.

The gene-trap vector used (pGT0, 1, 2tm-pfs) contained a splice-acceptor sequence, en-2, upstream of a transmembrane domain that is fused in frame with the reporter gene/selectable marker βgeo (itself a fusion protein with β-galactosidase and neomycin phosphotransferase II activities) [20].

3, 4 A foreign gene, such as a reporter or marker gene, is successfully incorporated into some viral genomes, including HIV‐1 and hepatitis C virus without the loss of replication competency.