Your English writing platform
Free sign upSuggestions(1)
Exact(14)
In this study, we used the reporter function of miRNA sponges that have the advantage of reporting miRNA activity instead of expression.
Exploiting the endogenous fluorescent properties of the hNET substrate ASP+, it was possible to simultaneously, and efficiently, sort pure cell populations and evaluate reporter function.
Adequate discrimination between specific and non-specific ASP+ staining is essential to study efficiency of hNET encoding vector transduction and reporter function and to facilitate isolation of pure hNET positive cell populations.
Here we explored CD19cre, CD4cre and LysMcre mice to activate the reporter function in B cells, T cells, monocyte/macrophages and neutrophils.
Using cell specific reporter function and conditional deletions we could define cells expressing LysM as the major IFN-β producers, with cells formerly defined as Tip-DCs being the highest.
The reporter function relies on the miR-9-induced GFP downregulation of the sponge transcript.
Similar(46)
To combine the advantages of both reporter functions, representatives of both types were jointly encapsulated in a sol gel matrix and immobilized onto a glass surface, to generate a bioluminescent toxicity and a fluorescent genotoxicity sensor.
Recent advances in the field include the expansion of available reporter functions with multicolored fluorescent proteins, a broadening of the detected chemical effects such as the availability of nutrients and enhancement of the spectrum of reporter microorganisms to include cyanobacteria, yeast and fungi.
This CFP CL-1 reporter funCFP CL-1s a biomonitoreporteriquitin-related degradation whereby Cfunctionsscence levels ase low when the protein is degraded (i.e., UPS is active) and the fluorescence much higher when the protein is not degraded (UPS activity is functionally impaired).
We hypothesize that the GFP folding reporters function by a so-called 'folding interference' mechanism, involving the formation of non-productive, non-fluorescent trapped folding intermediates of the GFP moiety and fused test protein domains.
Having demonstrated high-level fluorescence using far-red reporters in E. coli and M. smegmatis, we wanted to determine if the same reporters functioned in other mycobacteria.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com