Sentence examples for reporter dna from inspiring English sources

Step (ii) Addition of reporter DNA.

The thiolated reporter DNA was complementary to the stem moiety at the 3′-end of the probe.

The CaCO3/CMC@AgNPs composites exhibited 3.6 times higher ECL intensity than the pure AgNPs-labeled reporter DNA.

Finally biotinylated reporter DNA is captured by the streptavidin and quantified by real-time PCR using FastStart universal SYBR Green Master ROXX) kit.

Quantum dots (QDs) functionalized with reporter DNA are thus specifically bound onto a capture probe-modified 96-well plate by one-step hybridization for simple RNA detection.

The assay employs magnetic beads (MB) modified with a capture DNA sequence and a reporter DNA probe labeled with the enzyme, both made to be highly specific for L. brevis DNA.

In comparison with conventional organic dye-modified reporter probes, the use of reporter DNA-modified QD conjugates increase the melting temperature and lead to the detection of short RNA without the need for a ligation reaction.

Zhang and co-workers modified Au NPs with two types of signaling reporter DNAs; one probe is complementary to the target DNA, while the other is not.

For generating lentiviral reporters, DNA fragments as described in Fig. 1A were obtained by PCR amplification from H1 hESC-derived genomic DNA template and cloned into pGreenZeo lentiviral vector (System bioscience).

However, the testing of this multi-reporter DNA construct by transgenesis does suggest that the linkage of two different genes together, despite their large size, can still create a positional effect.

Briefly, aliquots (approximately 5 μg) of pStable-MyoD 6.0-luc (or other derived reporters) DNA were mixed 1 5 with Lipofectamine™ (Invitrogen) in Hepes buffer (20 mM Hepes, pH 7.0, 187 mM NaCl, 5 mM KCl, 0.7 mM Na2HPO4 and 5.5 mM dextrose) in 1.5 ml tubes and incubated at room temperature for 10 15 min to allow DNA and liposome complexes to form.