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Green tea extract activates ARE-mediated reporter activity [16].
Reporter activity was measured using the BrighGlo kit (Promega).
Overexpression of G9a4KR also repressed the reporter activity (Fig. 2e).
Furthermore, chromatin compaction is predictive of reporter activity.
Sequential analyses showed myocytes increased Mef2-dependent reporter activity before death.
Consistent with previous reports17,22, overexpression of wild-type G9a significantly repressed reporter activity.
The reporter activity was expressed as arbitrary luciferase units (firefly/renilla).
As expected, miR-124 markedly repressed the luciferase reporter activity in both cells (Fig. 5D,E).
Reporter activity was analyzed with the Promega Dual-Luciferase® Reporter Assay System.
FGFR4 promoter-luciferase reporter activity increased (p<0.05) in the presence of FA (Fig. 2a).
M.L.S. designed lentiviral vectors for gene KO, overexpression and reporter activity.
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