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HMPV remains poorly characterized and has been reported to replicate inefficiently in vitro.
Recently some new exciting experimental models based on angiotensin II infusion have been reported to replicate the human diseases, such as postmenopausal hypertension, preeclampsia, vascular remodeling, vascular aging and neovascularization.
The similar IncX1 plasmid R485 has previously been reported to replicate at an average copy number of 3 5 plasmid copies per E. coli cell [24] indicating that the pX1.0 replicon operates at levels comparable to its natural equivalents.
HCMV has been reported to replicate at low levels in infected cells and to be reactivated upon immune suppression, especially in transplant recipients (Coaquette et al. 2004).
Cytomegalovirus (CMV) infection in transplant recipients is reported to replicate with a doubling time of 1.2 2 days, and weekly screening is recommended for early diagnosis.
Interestingly, ICP6-defective oncolytic herpes virus has previously been reported to replicate in quiescent tumour cells carrying p16 deletions independent of cell cycle status (Aghi et al, 2008).
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Given that reovirus has been reported to preferentially replicate in cells with an activated Ras pathway and that Ras is mutated in the majority of pancreatic cancers, we hypothesized that Reolysin may have significant activity against this tumor type.
Reoviruses are naturally occurring viruses that are non-pathogenic and have been reported to specifically replicate in cancer cells with an activated Ras pathway but not in normal tissue.
Reovirus has been reported to selectively replicate in cancer cells with an activated Ras pathway.
Reoviruses have been reported to selectively replicate in cancer cells harboring an activated RAS pathway [ 6].
Reovirus is an oncolytic virus that has been reported to selectively replicate in cells with an activated Ras pathway.
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