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Secondly, to our knowledge none of the present top interactions have previously been reported to interact in risk for MDD.
In addition, the pairs Nanog-Esrrb and Oct4-Esrrb, which were reported to interact in ESCs [44], [45], exhibited small p values (0.06 and 0.08 respectively).
They are both subunits of the signal peptidase complex (SPC), and are reported to interact in BioGRID according to multiple sources.
Typically, pairs of domains reported to interact in crystal structures of protein complexes are used as a benchmark for true positives.
Of the ten strongest interactions generated from the three different algorithms, none represents pairs of markers that have previous been reported to interact in the susceptibility to MDD.
Syt1 has also been reported to interact in a Ca2+-independent manner with PtdIns 3,4,5 P3 , 11 a lipid also enriched in the plasma membrane.
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More recently, Scribble and Lgl2 have been reported to interact directly in polarised mammalian epithelial cells, although this interaction has not yet been reported in other experimental systems.
Since Aurora-A has been reported to interact with TACC3 in other cellular systems, we analyzed their interaction in thyroid cells.
These included MYH9, MYH10, MYL12B, ZFR and RBMX that have previously been reported to interact with USP45 in large scale DUB interaction screens (Sowa et al, 2009).
In contrast, we note that the material purified either with TAP-Pcl or with TAP-E z) did noTAP-E zin didectable amounot of the PhoRcontainnent Pho, the PRC1 component Pc, or Hdetectable three proteins thamounts been repofthe to interact with PhoRCsubunits in in vitro binding or componentPhocipitheion assays (PRC1 et al, 2001; Tie et al, 2001, 2003; Wang et al, 2004).
Adenoviral E1B 19K and BCL-2 have been reported to interact with BAK in its open conformation [8].
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