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Others have reported spurious oligodendrocyte differentiation of hESC-derived NSC in vitro [11].
Some have reported spurious copy number changes in FFPE samples and generally FFPE samples fail more often than fresh frozen.
However, even with such precautionary measures, studies have reported spurious findings, such as the identification of four putative myxozoan Hox homologs [ 22] that were later shown to be of Northern pike and bryozoan origin [ 9].
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This may increase the risk of reporting spurious results or associations that occurred by chance.
Inappropriate treatment may result in reporting spurious expressed genes (false positives) and missing the real expressed genes (false negatives).
However, the likelihood of reporting spurious findings was minimized by using the Bonferroni correction and setting the P value at < 0.01.
A drawback of the attempt to identify as many factors as possible was the large number of analyses and the associated risk of reporting spurious results.
Despite this limitation, the integrative and knowledge-driven nature of our approach can aid in reducing the possibility of reporting spurious associations.
The recent report by the Wellcome Trust Case Control Consortium [ 2] discusses in detail the potential artefactual influences on copy number determination and demonstrates the potential for reporting spurious associations with CNVs, especially multiallelic regions.
These connections were identified using a manually curated list of KEGG pathways that excludes overly generic and largely populated pathways (e.g., Pathways in Cancer) that would report spurious relationships (Goel et al., 2014).
To avoid reporting spurious results, our simulations included careful tuning of hyperparameters to minimize model sensitivity, steps for assessing convergence of the algorithm and applied different normalization and preprocessing methods.
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