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The PCR primers were designed from GenBank reported sequences.
We designed and optimized primers according to the previously reported sequences.
The vp68 sequence was 100%% similar with the four reported sequences (Table 5).
The Mamu-A1⁎002 Mamu-A1⁎002complepitopedentisal in all reported sequencompletelyridenticalciniallcowpox and MPXV.
The nucleotide sequence of the gene encoding for envelope protein vp26 was compared with 16 reported sequences and it was revealed that there was 100%% similarity with fourteen of the reported sequences (Table 3).
Point mutations: T → C, T → A and C → T were found at six different nucleotide positions with 13 reported sequences.
Gene sequence analysis revealed that the merP gene showed 86 99% homology, while the merT gene showed >98% homology with previously reported sequences.
Recent transcriptomic studies of sea anemones have identified several novel candidate peptides, some of which have cysteine frameworks identical to those of previously reported sequences.
Combined with the reported sequences, these mouse motif sequences were compared with those of other species such as Saccharomyces cerevisiae and Caenorhabditis elegans.
The cDNAs encoding for interleukin (IL -2, IL -2feron (interferon12p35 and IFN γp40 were amplified using specific primers designed from reported sequences of bovine cytokine genes.
The cDNAs encoding for IL-4, IL-10 and IL-13 were amplified using specific primers designed from reported sequences of bovine cytokine genes.
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