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In addition to the reported localisation at focal contacts and lamellipodia, a perinuclear and nuclear distribution of the protein was observed.
Previously published studies in other cell systems reported localisation of both proteins in the trans-Golgi network [ 16- 19, 25, 26].
In the M. truncatula root, MtPIN2 is expressed in the root, our data also showed the expression of this gene (Mtr.45124.1.S1_at), specifically up-regulated in the root meristem, consistent with the reported localisation of the AtPIN2 protein, where its localisation directs auxin flow from the root tip into the elongation zone and is crucial in mediating the gravitropic response [ 46].
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Although three Arabidopsis FPGS genes encode compartment-specific isoforms, no experimental study reports localisation of rice FPGS isoforms.
When analysed by confocal microscopy, wild-type seipin showed the typical reticular distribution and co-localisation with calnexin, consistent with the previously reported ER localisation (Fig. 1e) [ 5, 13, 20].
On the other hand, the cytoplasmic localisation of PR70/B β1 is in contrast with the reported nuclear localisation of its truncated PR48 form [ 20], suggesting that nuclear import/export of PR70 isoforms may be a regulated event.
It has a reported centrosomal localisation (Zhong et al, 2006; Tibelius et al, 2009), while a nuclear localisation has been reported in immunofluorescence (Rai et al, 2006) and immunohistochemistry (IHC; Rai et al, 2006, Human Protein Atlas (http://www.proteinatlas.org)).org
Full-length, GFP-tagged wild-type and mutant SMARCA2 proteins were localised exclusively in the nucleus, but not in nucleoli, consistent with reported subcellular localisation [ 28].
While Golgi localisation of the endogenous protein and of an EGFP-Irgm1 αK construct apparently identical to our own [20] was reported, no localisation of either to a LAMP1 compartment was reported in uninfected cells.
This is consistent with the reported cytoplasmic localisation of CVH in avian germ cells [2].
This pathway is involved in pancreatic development and re-activation of this signalling system has been implicated in pancreatic carcinoma with reported nuclear localisation of the downstream effector β-catenin [25].
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