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At time 0 h, 3 h, 5 h, 8 h and 24 h, culture samples were acidified, extracted, and analyzed by GC-MSD as reported in Material and Methods.
The details of the cell counts estimation procedure are reported in Material and Methods section.
As reported in Material and Methods, none of these primary screens at single dose yielded any significant data to warrant a full-blown dose response study.
For edelweiss, apomixis has been reported in material from the Carpathians (Sokolowska-Kulczycka, 1959), while studies on material from the southern Alps suggested facultative apomixis (Maugini, 1962).
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The cell-permeant Sox2 protein was added 24 h after RNAi in the Sox2 siRNA group as well as incubator control embryos and it was renewed every 24 h as reported in Materials and Methods.
The construction of the series pMB-SV5 is reported in Materials and Methods section.
The mean lifetime, calculated as reported in Materials and Methods, is 1.53 ns.
Both MFC-U and MFC-C were fed with a digestate-based medium as reported in materials and methods.
These were identified using TaqMan® array human endogenous control cards (Applied Biosystems, Foster City, CA, USA) as reported in Materials and methods section and in Additional file 13.
However, we note that the observed patterns on experimental design are based purely on what was reported in 'Materials and methods' section.
The quality of our database was ensured by repeated external audits of the participating centres, as reported in Materials and methods.
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