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We designed a custom array gene chip that included approximately 650 top performing candidate genes identified by applying the supervised principal component survival analysis in each of the four previously reported datasets.
The total number of transcripts retrieved for C. reticulata transcriptome is congruent with previously reported datasets from other non-model species of invertebrates, including gastropods [ 29- 31].
> -wrap-foot> Curandon analysisysis of reported datasets from high-throughput studies is typically very different from SGD's regular curation duties.
This method involves the application of a discrete Fourier Transform on previously reported datasets of absolute power of brain signals delta, theta, alpha1, alpha2, beta1 and beta2 and the endogenous estrogen and progesterone levels along 28 days.
Although some studies have reported datasets comprising up to 34 leachate analyses, these are composites of much smaller datasets from different phases of prolonged eruptions spread over days or weeks (e.g. Chaitén, Durant et al. 2012; Eyjafjallajökull, Bagnato et al. 2013).
We are aware of potential sequencing errors affecting some of these datasets and have tried to disregard suspicious complete genomes or use corrected versions of the reported datasets (not necessarily those available in GenBank) [29], [41].
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1The authors are grateful to Ke Hu and Deliang Wang for the assistance in applying their algorithm [38] to the reported dataset.
The QSAR model developed showed satisfactory statistical parameters for the experimentally reported dataset (r2 = 0.9415, q2 = 0.8958, pred_r2 = 0.8894 and F-test = 112.7314), thus judging the robustness of the model.
The miRNA expression profiling is, to our knowledge, the first such reported dataset on the HapMap samples.
The reported dataset was obtained from homogenization of 145 hearts (∼20 µg protein) and further processing with a gel-LC-MS/MS proteomics approach.
All hits to the reversed H. salinarum protein sequences were also rejected and the false positive rate of the reported dataset at the peptide level is estimated to be 1%.
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