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There were fewer reported copies of the most popular 2009 strain in 2010, showing a modest relaxation of vaccination pressures.
The 2009 NA sequences are dominated by a single strain, which appeared around the world in several thousand identical reported copies.
In addition to the previously reported copies of DUXC in armadillo, cow and dog[ 1], we have identified closely related homologues in horse, dolphin and megabat (Additional file 4).
The majority of these single-copy gene families contain two sequences/contigs in A. americanum, though 12 families had three reported copies, two families had four, and two families had five gene sequences.
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In B. subtilis, pBAV1K-T5-luxABCDE replicates to 10 times the reported copy number of pGK12 in the same species [5].
The multiplex assays were repeated, without variation, up to four times for each sample, and real-time quantitative PCR reported copy number consistent with the ability to repeat the multiplex results.
Zhang et al reported copy number losses of mir-218-1 mir-218-1 mir-218-1n (16%), breand (36%) and melanoma (33%), however, the alternative precurSLIT2nd host gene were not mentinned [48].
This suggests that the 65000 to 38000 range in previously reported copy numbers [ 31– 33] reflects differences in antibodies and methodology.
Three studies have reported copy number changes in EOC [ 16, 17, 24] (Additional file 4: Table S2).
Furthermore, TOP2A gene locus at 17q21-22 has reported copy gains and overexpression, and regulated chemo-resistance in HCC [ 47].
The reported copy was 1,039-bp 1,039-bph 3-bp TIRs and imperfect 17-bp sub-TIRs and no coding capacity.
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