Exact(3)
The sensing system comprised three probes: capture probe H1, report probe H2, and report probe H3.
Capture probe was immobilized onto the surface of Streptavidin-enwrapped magnetic beads (SA-MB) through the force between biotin and avidin, which hybridized with the target bacterial DNA sequence partly, and the remaining connected with the report probe decorated AuNPs with DTNB and SH-DNA (AuNPs@DTNB@DNA).
When multiple probes were mapped onto the same gene, the probe with the highest expression level was used as the report probe for this gene.
Similar(57)
Experiment (g) also shows a much higher DPV response compared with DNA electrodes in the presence of ncDNA and reported reporter probe (experiment (d)), in mismatched DNA and reported reporter probe (experiment (e)),, and target cDNA with no reported probe (experiment (f)).
The report probes were labeled with fluorescein isothiocyanate (FITC).
Without Hg2+, the report probes were stable as molecular beacons in solution.
Herein, the fluorescence signal was magnified by capturing more report probes.
In our design, thiol and biotin modified DNA tetrahedral nanostructures were used as capture and report probes respectively.
In the presence of Hg2+, the T-rich capture probes and report probes will hybridize into double-helical DNA domains with the aid of T Hg2+ T coordination chemistry.
At the same time, the report probes which are original hybrid with the anchoring aptamer fragments on the AuNPs surface can be released and recovers the fluorescence.
Separating the target and captured report probes from reaction solution was benefit to decrease the background signal and interference from other metal ions.
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