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On the other hand, only one journal published a report for determination of the studied mixture using TLC-Densitometric and derivative ratio spectrophotometric methods [6].
There has been few report for determination of bioactive compounds in water extract of He-Shou-Wu [21, 22], but LC analysis of hydrophilic compounds is still a challenge.
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However, the spectrophotometric methods that have been reported for determination of ATR-Ca in its pharmaceutical formulations [10 16] suffer from major drawbacks.
However, the UV-visible spectrophotometric methods that have been reported for determination of ATR-Ca in its pharmaceutical formulations [11 17] suffer from major drawbacks.
The literature survey reveals that several methods have been reported for determination of NLX mainly including high performance liquid chromatography [3-7], high performance liquid chromatography coupled with mass spectrometry [8-11], gas–liquid chromatography [12], chemiluminescence [13-15 [13-15
A survey of literature showed that several analytical methods have been reported for determination of DL in its pure form, pharmaceutical preparations and biological fluids, where DL is not determined officially in any pharmacopoeia.
One LC MS/MS method using the negative ion mode has been reported for determination of GCK in human plasma, but in our hands, the response of the [M−H]− ion was negligible.
Cavalcante and colleagues [8] recently reported a case-control study for determination of the perioperative risk of narcoleptic patients undergoing general anesthesia compared with matched control patients (n = 76 each).
Besides, there is no any report for the determination of trigonelline and theobromine in green coffee beans by FT-IR spectroscopy.
Furthermore, there is no any report for the determination of trigonelline and theobromine in green coffee beans by UV VIS spectroscopy.
It is the first report for the determination of metals and metalloids in a wide range of tobacco products and it will be useful for health and environment authorities in Pakistan and other countries of South Asia.
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