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Dual-luciferase report assays demonstrated that miR-140-5p miR-140-5p miR-140-5pα by bindirectly 3′-Utargets
In an earlier report, assays for the titration of thymus-independent IgM antibodies to E. coli LPS and the IgM and IgG response to the thymus-dependent antigen tetanus toxoid were described.
However, it is important to realize that 3′UTR luciferase report assays in heterologous systems, such as 3T3 fibroblasts or HeLa cells, will be rather inappropriate to give the cell-type specific effects revealed in our assays.
Labelled miRNA pull-down (LAMP) assay system or luciferase report assays add reporters or labels to miRNAs on the 3'-UTR of transcripts of interest, allowing the identification and the analysis of direct interaction regions among miRNA and its target gene [ 7].
Bioinformatics and luciferase report assays were used to identify the target of miR-143.
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Several other studies have reported assays with high nil control responses [19], [36].
The apparent increased toxicity of the smaller Ag-NPs is in agreement with published reports assaying the size-dependent toxicity of Ag-NPs.
Different assays, including bioinformatics algorithms (TargetScan and miRanda), luciferase report assay and Western blotting, were used to identify the target gene of miR-638 in GC.
Activities of the firefly luciferase and Renilla luciferase in a single sample were measured sequentially using the Dual-Luciferase Report Assay System (Promega, Madison, Wisconsin, USA).
This was confirmed through luciferase report assay.
Please also note and report assay activity (=FDG activity) and assay time (=activity calibration time).
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