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Different assays, including bioinformatics algorithms (TargetScan and miRanda), luciferase report assay and Western blotting, were used to identify the target gene of miR-638 in GC.
Activities of the firefly luciferase and Renilla luciferase in a single sample were measured sequentially using the Dual-Luciferase Report Assay System (Promega, Madison, Wisconsin, USA).
This was confirmed through luciferase report assay.
Please also note and report assay activity (=FDG activity) and assay time (=activity calibration time).
Luciferase report assay of AP-1 activation was also applied to confirm the results.
AR transcriptional activity was measured by Cignal report assay kit (SA Bioscience, Frederick, MD, USA) as per the manufacturer's instructions.
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Dual-luciferase report assays demonstrated that miR-140-5p miR-140-5p miR-140-5pα by bindirectly 3′-Utargets
Bioinformatics and luciferase report assays were used to identify the target of miR-143.
However, it is important to realize that 3′UTR luciferase report assays in heterologous systems, such as 3T3 fibroblasts or HeLa cells, will be rather inappropriate to give the cell-type specific effects revealed in our assays.
PY extraction from semi-solid devices approached 100% efficiency demonstrating that the reported assay is suitable for evaluating stability of novel dosage forms intended for ALA delivery.
The lower detection limit of the reported assay was 10 parasites per ml in all seeded samples tested and considered highly satisfactory for diagnosis of Leishmaniasis in blood samples.
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