The different types of assays targeting classical bacterial functions and essential pathways, including DNA replication, cell wall biosynthesis, and protein biosynthesis, have been extensively described in recent papers [ 63, 64].
While some residual replication or cell wall formation may be occurring during infection, the cessation of mycolic acid assembly early in infection is consistent with evidence that the bacteria can lose its acid-fast property within the host [37].
The conditions studied (stationary phase, osmotic, oxidative and antibiotic stresses) greatly affect both the bacterial replication and cell wall synthesis, and therefore fast regulation of the genes involved in these processes is essential.
The majority of the essential genes code for proteins involved in basic cellular process such as translation, cell wall biogenesis, replication, recombination and repair, and transcription.
This bellow had outer diameter 22.0 mm, inner diameter 15.2 mm, and length 65.0 mm with a welded 40 mm diameter flange and a tiny cap, 0.12 mm double layer thickness wall, 19 replications, and a 13.5 N/mm spring constant.
Replication of treponemes in the wall of the aorta may lead to aneurysm, aortitis or aortic endocarditis.
Other accessory functions, such as DNA replication (node 6310) and cell wall remodelling (node 30242) are also shown.
The majority of first- and second-line TB drugs target the processes of cell wall metabolism, DNA replication, or protein synthesis within the cell.
With rigorous uncertainties incorporated, subtle, but biologically relevant, antibiotic-induced changes become directly quantifiable, even as these antibiotics target widely varying biological pathways including DNA replication, protein synthesis, or cell wall synthesis.
Although molecular functions of the Spt16-M domain are not known, mutations altering residues within this domain have resulted in phenotypes indicative of transcription initiation and elongation defects, defects in replication, and defects in cell wall integrity, indicating the functional significance of this domain (Myers et al. 2011; O'Donnell et al. 2009; Stevens et al. 2011).
