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Eight studies validated their findings further in internal independent replication samples, while another two studies replicated findings of significant associations from proceeding EWASs [ 16, 22].
As with other phenotypes, the first GWAS findings for imaging phenotypes were difficult to replicate, probably due to the limited availability of replication samples.
GWAS research with replication samples is valuable to establish robust evidence of a main genetic effect.
This variant did not remain significant when additional replication samples were examined.
The replication samples derived from two population-based Dutch cohorts and one Australian Twin study totaling 2,957 migraineurs and 5,774 non-migraineurs.
For the meta-analysis of discovery and replication samples the Cochran Mantel–Haenszel (CMH) association analysis with a significance threshold of p ≤ 5 × 10−8 was used.
Few "replication" samples manifest all of these features.
None of these were confirmed in replication samples (KORA S4, HYPEST, BRIGHT).
Combining our independent replication samples (AREDS B and BMES) yielded an OR = 1.87 (95%CI 0.83 4.02, 1-sided P≤0.064).
Genotyping was carried out using Sequenom (San Diego, USA) iPlex technology at Sequenom facilities in San Diego, USA Associationn samples) and Hamburg, Germany (Replication samples).
We observed further associations between genetic variants in DCLK1 and cognitive abilities in the replication samples but with differences regarding the panel of markers that were associated.
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