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We determined the complete replication program of C. glabrata using two complementary approaches, deep-sequencing of synchronized replicating cells and ARS capture.
The DNA replication program is, in part, determined by the epigenetic landscape that governs local chromosome architecture and directs chromosome duplication.
In this review, we summarize recent findings and current models linking spatial and temporal regulation of the replication program with epigenetic signaling.
The flexibility observed in the replication initiation landscape might help achieve complete and accurate genome duplication while coordinating the DNA replication program with transcription and other nuclear processes in a cell-type specific manner.
Here, we provide a complete analysis of the nucleosomal landscape and replication program of the human parasite Leishmania major, building on a better evolutionary understanding of replication organization in Eukarya.
Why a replication program exists at all is not clear.
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Replication programs are cell-type-specific (Letessier et al., 2011), and CFSs have not been extensively mapped in HEK293 cells.
Once the population density expands to critical threshold levels, QS systems activate replication programs and the full expression of virulence programs.
Under the assumption that the replication programs in C. albicans and C. dubliniensis lineages have remained similar after their divergence (i.e., considering that orthologous genes have the same t rep in the two species), comparable variations are observed when plotting the R t ratio of transitions occurring in the C. dubliniensis lineage as a function of t rep (fig. 5 B).
Recently, B-Myb was shown to stimulate G1/S transition independently of its sequence-specific DNA-binding activity and to affect the DNA-replication program, further highlighting the complex manner of cell-cycle regulation by B-Myb.
According to this model, local variations of GC3 are linked to temporal variations (averaged over evolutionary time) of, the proportion of dCTPs and dGTPs at time t rep when the sequences are replicated (considering that the replication timing program and the variations of the relative concentrations of dNTPs as functions of t rep have been globally stable).
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