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Then the replication products of long duplex DNA probes were stained with a sensitive reagent SYBR Green I (SG).
We describe the development of a single-tube RT-PCR assay to differentiate PCV2 replication products and virus DNA.
APOBEC3G (A3G) is a host-expressed protein that inactivates retroviruses through the mutagenic deamination of cytosines (C) to uracils (U) in single-stranded DNA (ssDNA) replication products.
At low levels of cleavable complexes, when only one replication fork is arrested, continued replication by the other unimpeded fork on the same plasmid DNA leads to the formation of linearized replication products.
rAAV-specific replication products were detected with the aid of the 0.7-kb NcoI×Eco52I fragment from pAAV.DsRed, which includes the entire DsRed.T4 ORF.
In a study of the changes in steady state levels of mRNA, cRNA and vRNA over a 24 hour period at 42°C remarkable differences between the levels of mRNA and replication products (vRNA and cRNA) were observed in the WSN strain, but not in the HK and SW strains tested (Figure 2C).
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The final replication product just has to be specifically recognized for genome packaging [ 12].
Taken together, these results indicate that the IREF-2-dependent unprimed vRNA product is a bona fide unprimed vRNA replication product from the cRNA template.
They used an RNA template of which 3'-terminal sequence is ---GAUCCAAGCUUACGU ACGCG-3' (the sequence showed in italics is a part of cutting site of MluI restriction enzyme) and got a double length replication product.
Leydig cell production of mRNA and protein for interferons (IFNs) and of three antiviral proteins 2′5′ oligoadenylate synthetase (2′5′OAS), double-stranded RNA-activated protein kinase (PKR) and MxA was investigated, in the absence or presence of mumps virus or viral stimuli including poly I C, a mimetic of RNA viruses replication product.
In experimental latent infection of granulocyte-macrophage progenitor cells in culture that do not express productive viral replication gene products, the cell surface level of MHC II is markedly reduced, synthesis and processing occur normally, and MHC II proteins together with HLA-DM are redistributed and retained within punctate cytoplasmic vesicles.
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