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Here we have investigated the replication pattern of the random asynchronously replicating genes in undifferentiated human embryonic stem cells, using fluorescence in situ hybridization based assay.
Although yeast telomeres are among the latest-replicating regions in the genome, this replication pattern is dependent on telomere length and telomeres in the shorter length range are replicated by early-firing subtelomeric origins [ 10, 11].
If h m < DT, it is in the replication pattern.
Packets are forwarded in the replication pattern at first after they are generated.
Fig. 3 Number of duplication hops growing in the replication pattern.
The approach discussed below aims at monitoring the replication pattern and the kinetics of replication throughout the entire genome of living cells.
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The primary and secondary factors as well as replication patterns need to be ascertained such that the impact of variation among the entities is minimized.
The variability in replication patterns is seen for example, on the X chromosomes in female mammalian cells.
This has not been described in the only other study that compared HHV-8 oral replication patterns among HIV-infected persons with or without KS [35].
We observed robust expression and nuclear localization of the Rbf1N-RFP in follicle cells, however BrdU labeling of follicle cells did not reveal any detectable changes in DNA replication patterns during endoreplication or chorion gene amplification (data not shown).
HHV-8 replication patterns, as measured by detection of viral DNA from samples collected from mucosal surfaces, have been defined in several longitudinal and cross sectional studies, with virus predominantly detected from the oral mucosa [11], [12], [13].
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