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After replication onto SC-Arg-Uri plates, the colonies were screened by colony PCR for the absence of the wild-type allele and the presence of the UAU1 insertion allele.
Additionally, if changes in nucleotide skew on the major strand are due to inversion of the control region or origin of replication onto the minor strand, then recombination must have occurred.
Selected numbers of single colonies from the last round of positive selection were screened by replication onto plates with varied concentrations of chloramphenicol (34, 50, 75, 100, 150, or 250 μg/mL) in the presence and the absence of the appropriate unAA.
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The originals of most of the works hang in museums and private collections, so Perzan and her team print and stretch replications onto canvas to be hung in the homes and offices of the Lyon family.
The ability of p16INK4a to inhibit the cyclin D-dependent kinases also enables it to block the assembly of DNA replication complexes onto chromatin and thus inhibit DNA replication, a function not shared by p21Waf1 [ 50].
Interestingly, RNAi targeting TopBP1 led to defects in the loading of replication factors onto DNA demonstrating a mechanism whereby knockdown of genes that function downstream of Cdk2 can result in a G1 arrest phenotype that persists in the presence of active Cdk2.
Replicative helicase is loaded onto replication origins at the initiation of DNA replication.
Two genes, ESCO1 and ESCO2, both encode acetyltransferases that acetylate the SMC3 subunit of cohesin during DNA replication to lock the cohesin ring onto DNA.
How the pol-prim complex is brought onto replication origins remains to be elucidated.
Crystal structures show how the sliding clamp that facilitates processive replication is loaded onto DNA.
A possible explanation is that lower stability and disturbance of local conformation of the pri2 mutant proteins compromise loading of the pol-prim complex onto replication origins.
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CEO of Professional Science Editing for Scientists @ prosciediting.com