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This has at least three reasons: (i) we expected a high false positive rate due to the relatively small screening cohorts in contrast to the number of measured epigenetic features, (ii) we performed the replication measurements with a completely independent methodology, and (iii) we carried out these analyses in independent patients and controls.
Using the experimental paradigm of this study, replication measurements with two participants were performed.
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In terms of these measurements of CA and replication measure (R), we were able to confirm that the UV NIL could be a possible tool to produce a hydrophobic film by replicating surface of plant leaves.
There were two treatment levels (control versus induced), three replications per level (3 culture wells per treatment) and two sub-samples per replication (duplicate measurements of absorbance values).
Limitation of the study: The authors acknowledge that the experiment suffers from lack of replication of measurements of ROS levels under the different light regimes.
We observe greater variation in the number of detected N signals for the cell lines compared to the reference, indicating that biological variation is larger than variation resulting from technical replication of measurements (Table 1).
Finally, replication timing measurements in PHA-stimulated lymphocytes provide potential blood indicators that would go along with PSA testing in identifying patients who should undergo biopsy to verify their cancer status.
Our investigation has many strengths, including large sample size, an independent population for replication, and measurement of serum retinol using the accepted, gold standard assay method across studies.
For each of the 8 feet and each of the 5 replications, the measurements consisted of the values for force and peak pressure for 120 points in time.
First, it is unbiased in the sense that it can be applied to any quantitative trait and any genetic polymorphism, much like traditional genetic mapping methods; in fact it can even be applied to previously published data sets, where appropriate replication of phenotype measurements in genetically identical individuals exists.
Within each of the experiments included in this analysis, we have accounted for biological variability by independent replication of the measurements using RNA from individual animals.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com