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The scientific community should establish a replication index as a measure of output quality.
The endpoints analyzed included: mitotic index (MI), replication index (RI), sister chromatid exchange (SCE) and chromosomal aberrations (CA).
Replication index was defined as the increase of copy number of progeny viral DNA compared to the copy number obtained 2 hours after transduction (set to 1).
The replication index (RI) was calculated by applying the following formula: RI = OD at 24 hr or 72 hr/OD at 0 hr.
To facilitate comparison between cultures, these Trep values were converted to replication indices (Friedman et al. 1996) by assigning ARS305 a replication index (RI) of 0 and R11 a RI of 1.0.
No significant differences were observed between unstimulated and IL-4/CD40L-stimulated cells with respect to either the number of divisions undertaken by responding cells ("proliferation index"; not shown) or the fold-expansion of responding cells ("replication index"; not shown).
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As expected, the replication indices for chromosomal ARS1 fragments were similar between the two strains (RI = 0.35 and 0.36).
We compared the average values of replication indexes [ 17, 29, 30] of origins containing different numbers of Forkhead-binding sites.
Next the average values of replication indexes (RIs) [ 17, 29, 30] were assigned to origins and the RI values of each group were plotted.
"Index replication is the ultimate goal, but if you can do it in a more tax advantageous way for the first four to five years than being in a pure index fund, that gives you more tax flexibility going forward".
Items 8 and 9 on the QUADAS checklist are concerned with replication of the index and reference test.
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