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10.7554/eLife.07218.004 Figure 2. Atypical and classical MBCs are similar in isotype distribution, replication history and Ig repertoire characteristics.
Hence, two among these three systems specifically examined for the existence of DNA-strand based mechanism proDNA-strand based cell division through DNA structure and the replication history of DNA strands at the specific locus.
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These memory subpopulations show different frequencies of somatic mutation and different replication histories that are thought to reflect their formation on primary or secondary germinal centres or outside germinal centre reactions [ 5].
B cells were then FACS-sorted to obtain naïve B cells, classical and atypical MBCs and the replication histories were determined using a qRT-PCR-based assay that measures κ-deletion recombination excision circles (KRECs) (van Zelm et al., 2007).
Thus, atypical and classical MBCs share similar isotype distributions, replication histories and Ig repertoire characteristics, which taken together, suggest that atypical and classical MBCs are derived from a common precursor and differentiate in response to similar antigen-dependent selective pressures.
To determine the in vivo replication histories of the three B cell subpopulations, B cells were purified from peripheral blood mononuclear cells by removing all non-B cells and plasma cells/plasmablast yielding B cells of >98% purity.
In light of the numerous failures of replication in history of molecular genetic studies of intelligence [73], efforts at replication and extension would also benefit from a larger sample size.
Two of Dr. Holmes's other highly acclaimed books are "Meselson, Stahl, and the Replication of DNA: A History of the Most Beautiful Experiment in Biology" (2001), and "Lavoisier and the Chemistry of Life" (1985), a study of the scientist often referred to as the father of modern chemistry.
The basic idea of replication has a long history in biology.
This hierarchy fits the actual evolutionary history of replication and development even better.
2. The history of replication of a genetic element without recombination is isomorphously represented by a directed tree graph (an arborescence, in the graph theory language).
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