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These observations are consistent with the hypothesis that RECQL4 has one or more cellular functions in addition to its role in initiation of replication, functions which are dependent on the a functional helicase domain.
To test whether this reduction correlated with suppression, the doubling time of 20 metabolic mutants, WT for replication functions, was measured at high temperature in LB.
In the current mutagenesis experiments we aimed to use the large number of point mutants to separate genetically the complementing movement and replication functions.
To determine if MRE11 and NBS1 contribute to OriP replication functions, we assayed the effects of siRNA depletion on OriP plasmid replication (Fig. 2).
In combination with our data that Smc3 acetylmimetics fail to bypass ctf7 cell8 celethalityity, we propose a more plausible scenario in which Ctf8-RFC, in addition to exhibiting roles DNA replication, functions in cohesion separately from Ctf7 (Figure 10).
In the case of replication, full viability is restored to mutants affected in 3 DNA elongation factors out of 9 replication functions tested (3 specific for DNA initiation and 6 mainly involved in DNA elongation).
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The simulation process stops if it reaches the specified number of generations, or if the coding systems stop replicating, e.g. if the replication function ρ(χi(T)) = 0 for every coding system χi in the population at generation T.
The number of offspring coding systems generated by each coding system in a population within a single generation is computed based on the replication function ρ (see subsection "Replication function").
Taken together, our present data indicate that Swi3 has a replication function that is independent of Cds1 activation.
The parameter k defines the contribution of the effect of the size deviation to the replication function.
In contrast, the C-terminal domain of p21Waf'1/CIP1, which can bind and inhibit the DNA replication function of proliferating nuclear antigen (PCNA) is unique [7], [8].
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