Successive steps were used to prioritize loci for replication, assessing the association of these loci with capillary glucose and repeated measures of multiple complications of diabetes.
The process of developing treatment-based subgroups can be divided into 3 stages: 1) hypothesis generation; proposal of potential effect modifiers; 2) hypothesis testing; testing of the potential effect modifiers; 3) replication; assessing generalizability [ 2].
The 60 CD-1 mice used in this replication were assessed (in 2 independent replications) on five learning tasks (i.e., the Lashley III maze, passive avoidance, spatial water maze, associative fear conditioning and odor guided discrimination) which have previously constituted the core tasks used to evaluate general learning abilities.
At one week post infection with Ad5-CRAD, CAR was undetectable in both GFP+ cells (with viral replication) and GFP- cells (without viral replication) as assessed with RmcB mAb (Figure 2A).
As each probe was spotted as four replicates on the arrays, within-array replication was assessed using the limma duplicate correlation function.
Virus replication was assessed during primary infection of rhesus monkeys by measuring plasma SIVmac p27 levels and by quantifying virus replication in lymph nodes using in situ hybridization.
Replication testing of seven SNPs revealed one SNP in ABL1 to have an association in one of the four replication populations assessed (also from the US) and was significant overall with a recessive model.
Parasite replication was assessed by light microscopy [51].
After five days, residual virus replication was assessed by measuring HA activity in the culture supernatants.
Parasite development and replication were assessed in cultures by microscopic examination of Giemsa-stained thin blood smears, as reported before [31].
