Similar observations were also made in our earlier study of colorectal carcinoma (Scott et al, 2003), in which cyclin A expression was also not detected in any cell not seen to be actively replicating DNA using our in situ replication assay (Mills et al, 2000).
We transfected the SHM vectors into cells that do not express the T antigen to prevent the plasmids from replicating, which was confirmed by a DpnI replication assay (data not shown).
Compound 4 was also tested as potential anti-HCV compound in a subgenomic replication assay.
A single NS5B M31I substitution enhanced replication to levels sufficiently robust to quantify sensitivity to HCV inhibitors in a transient replication assay.
Using a high throughput primary β-cell replication assay, two adenosine kinase (ADK) inhibitors, 5-Iodotubercidin and ABT-702, have been identified and shown to increase β-cell mass (Annes et al., 2012).
This work describes a novel HCV replicon sustained by a genotype 2b polymerase that is sufficiently robust for quantifiable analysis in a transient replication assay, and demonstrates its utility in characterizing anti-HCV compounds for cross-genotypic efficacy.
The linear dipeptide compound 1 and the non-peptidic fused ring compound 2 show comparable activities against DENV-2 NS2B-NS3 protease and wild-type DENV-2 virus in a viral replication assay.
In a previous study, the DNA polymerase gene (dnapol) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) was identified as one of six genes required for plasmid replication in a transient replication assay (M.
As someone who has trouble working a washing machine, I consider it a personal achievement that I am able to use a confocal microscope, run a Western blot, and carry out a DNA replication assay!
The AAV DNA replication assay was performed as described previously [25].
The replication assay was performed following the manufacturer's instructions (Click-iT EdU Alexa Fluor 488, Invitrogen).
