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Berry et al. report that impact 'is assessed in terms of visitor numbers and characteristics, experiences, and replication activities that emerge from engagement with an eco open home event' (([17], p. 423); see, in a similar vein, [18]).
It is thus possible that in the chromosomal context the replication activities of close ARS and NUMTs are additive.
To further confirm the MTT data, DNA replication activities were examined by analyzing incorporation of BrdU in cells transfected with Akt2 siRNA.
Next, replication activities of these 3'NTR mutated replicons were compared by monitoring colony forming activities in transfected cB76.1/Huh7 cells.
Five of these predicted replication origins in Haloarcula hispanica were experimentally confirmed via autonomous replication activities.
To confirm these putative replication origins, we performed a genetic assay to test their autonomous replication activities.
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This ratio of additional foci to replication activity is consistent with the injected Cdc45 activating 3-fold the number of preRCs, and thus producing 6-fold the number of forks, that would otherwise have been active.
The increased replication activity could be due to faster fork movement or additional preRC/replicon activation, or both.
Last, the cell DNA replication activity was examined using BrdU, a synthetic thymidine analogue that binds to replicating DNA.
There might be a mutual influence on replication activity of HBV and HCV.
Cytotoxicity, anti-HBV antigen secretion activities and anti-HBV DNA replication activity were assayed with cell counting kit-8 (CCK-8), enzyme linked immunosorbent assay (ELISA) and a real-time PCR, respectively.
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