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"We can't keep replicating the same tactic and expect the same level of success.
As shown in Figure 2A (proteins) and 2B (peptides), the three replicates of each method clustered together, consistently with a higher similarity among replicates of the same method than among different methods; moreover, when grouping samples into two clusters, DT data clustered separately from FASP-ISD both at protein and peptide level.
Repeat the same method with back sloper.
We sought to determine whether these findings could be replicated using the same methods and a different data source.
Because the previous study was the first large epidemiologic study to address risks of adverse events associated with intravenous iron use, we sought to determine whether these findings could be replicated using the same methods with data from another U.S. dialysis chain where the patient demographics, case mix, and other aspects of medical practice may differ.
The problem: The best evidence for a genuine discovery is showing that independent scientists can replicate it using the same method.
Replicate preparations using the same method are the next lowest in variability, again with same day hybs being less variable than different day hybs.
The proposed inter-methods agreement coefficients compare the disagreement between measurements made by different methods on the same subject to the corresponding disagreement between replicated measurements made by the same method.
The multiple readings within a subject may be replicated readings by using the same method or/and readings by using several methods (e.g. different technologies or several raters).
RNA for qRT-PCR analysis of the other two biological replicates was extracted by the same method, except 200 mg of ground grape tissue was used and volumes were adjusted accordingly.
We compared the RMS deviations between pairs of hybridizations performed using the same total RNA prep (hybridization replicates) with those performed using different total RNA preps done with the same method (prep replicates), and also with those using different total RNA prep methods.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com