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Metabolism was similar between the two replicating stages.
8323 genes were annotated with sequence reads across the two asexually replicating stages of the parasite life cycle.
Subsequent malaria smears and qPCR remained negative and no evidence of asexual or replicating stages were found.
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False-positive determinations of activity against nonreplicating M. tuberculosis may arise from carryover of compounds from the nonreplicating stage of the assay that act in the replicating stage.
There has previously been an attempt to parallelize the MT19937 algorithm by dividing the seeds into various pools and then replicating the stages 2 and 3 to generate multiple outputs; see [7].
Late asexually replicating parasite stages sequester away from the bloodstream deep in host tissues, and this process is linked to organ-specific pathology such as cerebral malaria and pregnancy-associated disease.
The study consisted of 2 procedurally identical replicate stages, stage I and stage II.
PCNA has previously been shown to form distinct DNA replication foci in the parasites during replicating trophozoite stage.
Because the nonreplicating assay is bacteriostatic and coupled to a replicating outgrowth stage to permit surviving cells to increase in biomass, a replicating-inactive drug must be nonreplicating-bactericidal to give an NR-MIC90 (Fig. 1).
Approximately one-third (29/85) of the most significant non-MHC SNPs in Stage 1 (Table 1) had original GWAS P-values <0.10 in both the TDT and CMH tests, with only two of these SNPs further replicating in Stage 2 (rs11583328 and rs10469900) (Table 2).
Chemotherapy of symptomatic toxoplasmosis is mandatory; however, available drug regimens including pyrimethamine plus sulfadiazine, clindamycin, and atovaquone are limited and are only effective against the replicating tachyzoite stage [ 9].
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