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The under-age teams, for instance, were replicating set moves regularly used by the first team.
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There were two replicates set up in each rearing environment.
Before proceeding to microarray analysis each replicate set was checked for effective FGF inhibition.
Each replicate set comprised control, dnFGFR1 injected and dnFGFR4 injected embryos.
Under these methods all characters were weighted equally and bootstrap replicates set to 10,000.
Linkage analysis was conducted as described above for each replicate set.
Sibling embryos from each replicate set were analysed for dp-ERK levels and expression of Cdx4, Brachyury and MyoD by both RPA and in situ hybridization.
Each replicate set consisted of control embryos and embryos injected with dnFGFR1 or dnFGFR4 collected at stage 10.5 (11 hours pf at 23°C).
Replicate sets of each of the mutants were compared to the RPE1 replicate set.
maximum likelihood method with bootstrap replicates set as 1000.
Biological replicates (set I and set II, where available) were combined to increase read coverage.
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