Sentence examples for replicating sequences from inspiring English sources
To elucidate the role of the chromatin environment in the regulation of replication origin activation, autonomously replicating sequences were inserted into identical locations in the budding yeast genome and their activation times in S phase determined.
Saccharomyces cerevisiae differs somewhat in that specific DNA loci that function as replication origins (termed Autonomously Replicating Sequences or ARSs) contain a consensus sequence (ARS Consensus Sequence or ACS) that is essential for ORC binding and origin function [ 1, 20, 21].
This property allows for precise identification and manipulation of replication origins, also known as autonomously replicating sequences (ARSs).
Eukaryotic DNA replication is initiated from multiple origins that are defined as autonomously replicating sequences (ARSs) in budding yeast.
As replicating sequences grew longer, new, more complex functions or faster catalytic activities could have been accessed.
Although it is well known that yeast autonomously replicating sequences (ARSs) bind nuclear matrix and MARs also function as ARS elements in yeast, whether a heterologous MAR or ARS element acts as a replication origin in the chromosome has not been elucidated.
Furthermore, we demonstrated that autonomously replicating sequence plasmids with H. hispanica minichromosomal replication origins were extremely unstable.
TAR cloning requires that the cloned DNA fragment carry at least one autonomously replicating sequence (ARS) that can function as the origin of replication in yeast.
They contain the yeast selectable marker URA3, the negative-selection marker CYH2 [40], a yeast centromere and an autonomously replicating sequence (ARS).
ARS, autonomously replicating sequence; 5-FOA, 5-fluoroorotic acid; TAR, transformation-associated recombination; YAC, yeast artificial chromosome.
The complex binds to the origin sequence called autonomously replicating sequence (ARS) in chromosomes of budding yeast.
