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In addition to the lac operon that is bound by the lac-IZ column during the minichromosome isolation, the plasmid also contains the TRP1 selection marker and the autonomously replicating sequence ARS1, necessary for plasmid replication [22].
Furthermore, we demonstrated that autonomously replicating sequence plasmids with H. hispanica minichromosomal replication origins were extremely unstable.
TAR cloning requires that the cloned DNA fragment carry at least one autonomously replicating sequence (ARS) that can function as the origin of replication in yeast.
TAR cloning requires that the cloned DNA fragment carry at least one autonomously replicating sequence (ARS) that can function as the origin of replication in yeast, which prevents wide application of the method.
Hence, any of these nine ARSs would be a good choice for an efficient multicopy plasmid replicator in C. glabrata.> -wrap-foot>> -wraNotoot> aNot including rDNA repeat arrays kb kilobase pairs ARS autonomously replicating sequence, bp base pairs, nt nucleotides In S. cerevisiae, early replication origins exhibit enriched tridimensional contacts in the nucleus [ 43].
They contain the yeast selectable marker URA3, the negative-selection marker CYH2 [40], a yeast centromere and an autonomously replicating sequence (ARS).
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This property allows for precise identification and manipulation of replication origins, also known as autonomously replicating sequences (ARSs).
Eukaryotic DNA replication is initiated from multiple origins that are defined as autonomously replicating sequences (ARSs) in budding yeast.
As replicating sequences grew longer, new, more complex functions or faster catalytic activities could have been accessed.
The experimental work indicated that the main chromosome and the three smaller chromosomes (pHV4, pHV1, and pHV3) all contain functional autonomously replicating sequences (ARS).
This has been demonstrated in Saccharomyces cerevisiae, where origins consist of DNA segments of ∼200 bp, also named Autonomously Replicating Sequences (ARS), as they confer to episomal plasmids the ability to replicate.
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