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Figures 3A and 3B show how each protein identified in the replicating sample (y axis) was redistributed into temporal FCM clusters after inhibitor treatment (x axis).
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Biological and technical (dye swap) replicate sample sets are analysed.
Replicate sampling plot (Replication), and replicate sampling plot nested within each sampling year (Year/Replication) were set up as the random effect for former and latter models, respectively.
In this study, five replicated samples were used from each treated and untreated sample.
CIs were computed as bootstrap 95% percentile intervals based on 10000 replicated samples.
The calculated error rate was approximately 3% based on negative controls and replicated samples.
This exceeds previously published metatranscriptomic studies because of the inclusion of replicated samples.
All the replicated samples exhibited identical results.
For 'Holiday', two replicated samples are presented.
Replicated samples for the same patient were averaged.
In our study, we had replicate samples on all participants.
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