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Instead, more emphasis is put on understanding the chemistry of the primeval syntheses and the mechanisms of information processing in primordial replicating cycles [ 17- 33].
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This demonstrates that the BoHV-4 replicating cycle is dependent on gB.
This observation suggests that the increase in SPREAD frequency requires the induction and/or synchronization of cells in the replicating cycle upon the second TPA treatment.
The SmartCycler II system is a PCR system by which amplification and detection are accomplished concurrently with TaqMan technology (Applied Biosystems, Foster City, Calif) using fluorescent probes to detect amplification after each replicating cycle.
Qiagen HCV quantitative kit was used to perform HCV RNA quantification with 10 ul of the extracted RNA on Roche Real Time PCR using fluorescent probes to detect amplification after each replicating cycle.
In the present study we have genetically engineered a fusion molecule encoding two chlamydial proteins; CT521 that resides in the cytosol of reticulate bodies during the replicating cycle (data not shown) and CT443 (omcB) located in the outer cell wall [41], [42].
In the case of pizza parlours, that might mean identifying and replicating cooking cycles, measures and how toppings are put on the crust.Mr Quinn and Ms Paquette coin the term "micro-management" to describe how service firms can benefit from concentrating on ever-smaller units of their businesses.
Having recorded all the numbered Sibelius symphonies with the Minnesota Orchestra, replicating the cycle that he first tackled in the 1990s with the Lahti Symphony, Osmo Vänskä now revisits Sibelius's first exercise in large-scale symphonic form.
They are obligate intracellular parasites that have two distinct replicating life cycle forms in the mammalian host.
Each replicate cycle threshold (Ct) was normalized to the average Ct of the two endogenous controls on a per sample basis.
Each replicate cycle threshold (Ct) was normalised to the average Ct of five endogenous controls (B2M, HPRT1, RPL13A, GAPDH and ACTB) on a per plate basis.
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