A significant challenge with the use of RT inhibitors is the development of resistance; because HIV replicates continuously at a very high rate, there are many chances for mutation and hence the emergence of a virus resistant to many drugs.
The project, funded by the Wellcome Trust, has used more than a hundred spare IVF embryos from fertility clinics to establish several embryonic stem cell lines that replicate continuously in the laboratory.
Cells from that biopsy became the first to successfully replicate continuously in culture (and hence be called immortal), and have since played a crucial role in many scientific breakthroughs.
Even during this period of clinical latency, the virus replicates continuously and causes new rounds of infection.
It has been suggested that the discontinuous replication of the lagging strand by the Okazaki fragments increases the frequency in which the template of the lagging strand is in single-strand (ss) DNA conformation relative to the template of the leading strand, which is replicated continuously (Frank and Lobry 1999).
In the above-mentioned field-based experiment, replicate plots continuously received either ambient or simulated increased atmospheric nitrogen deposition over a 14-yr period beginning in 1994.
In contrast, upon differentiation stimulus, the parasites slow down their replication rate continuously, until they stop replicating in mature cysts.
Nucleotide imbalances interfere with mtDNA but not nDNA replication, because post-mitotic cells do not replicate nDNA, but continuously turnover mitochondria and thus replicate mtDNA.
To establish a model of type II EMT, a continuously replicating line of human small airway epithelial cells was generated by immortalization using human telomerase (hTERT) and CDK4 expression [ 33].
We have previously validated a model of Type II EMT using a continuously replicating line of human small airway epithelial cells (hSAECs) immortalized with human telomerase (hTERT) and CDK4 expression [ 21].
