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The decision on the number of lots and replicates will be a balancing act between the uncertainty of the measurement, design and other practical considerations.
Since technical replicates of a single RNA preparation were used, this experiment captured only technical error, and biological replicates will be more variable.
The bootstrap replicates will be plotted in cost-effectiveness planes (CE-planes).
Each condition will be run once (i.e., no technical replicates will be performed).
For visualization of sheared DNA from 10,000 cells on agarose gels, 3 × 50,000 cell replicates will be required.
This is because the variability in measurements across replicates will be dominated by Poisson noise making estimates of non-Poisson variance unreliable.
Similar(48)
Any clinical features that can be measured and replicated will be considered.
SK-MEL-5: 2000 cells/well x 384 replicates Experiment will be done a total of once.
Considering an average mzML file of 3 GB in size and six replicates, we will be processing nearly 20 GB in the experiment.
Finally, for drugs that produce foci only when DNA replicates, it will be necessary to ensure that all treated cells have the opportunity to transit S phase.
If it can be replicated, it will be.
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