The robustness of each branch was estimated by non-parametric bootstrap analysis (1000 replicates) using PHYML.
The analysis was conducted in five replicates using new DNA electrode for each sandwich hybridisation assay.
Allele-specific expression levels were estimated in the hybrids between the two treatments in both replicates using DESeq.
Overall, we preformed three biological replicates and three technical replicates using the LightCycler® 480 II RT-PCR System (Roche, Switzerland).
All the experiments were developed in three replicates using cellulose conversion and ethanol yield as response variables.
The experiments were carried out (with 30 replicates), using a glass cage (2/l), covered with a soft cloth.
ML bootstraps were inferred from 300 replicates using the same parameters.
We calculated 95% confidence intervals with 1000 replicates using Arlequin's parametric bootstrap approach.
Total RNA was prepared from three independent biological replicates using TRIzol reagent.
Estimates were obtained for three independent replicates using a Bayesian framework.
Each extract was assayed in 4 6 replicates using different dilutions.
